| Registro completo |
| Provedor de dados: |
Nature Precedings
|
| País: |
United Kingdom
|
| Título: |
3D model of amphioxus steroid receptor complexed with estradiol
|
| Autores: |
Michael E. Baker
David J. Chang
|
| Data: |
2009-06-03
|
| Ano: |
2009
|
| Palavras-chave: |
Cancer
Developmental Biology
Bioinformatics
Evolutionary Biology
|
| Resumo: |
The origins of signaling by vertebrate steroids are not fully understood. An important advance was the report that an estrogen-binding steroid receptor [SR] is present in amphioxus, a basal chordate with a similar body plan as vertebrates. To investigate the evolution of estrogen binding to steroid receptors, we constructed a 3D model of amphioxus SR complexed with estradiol. This 3D model indicates that although the SR is activated by estradiol, some interactions between estradiol and human ER[alpha] are not conserved in the SR, which can explain the low affinity of estradiol for the SR. These differences between the SR and ER[alpha] in the steroid-binding domain are sufficient to suggest that another steroid is the physiological regulator of the SR. The 3D model predicts that mutation of Glu-346 to Gln will increase the affinity of testosterone for amphioxus SR and elucidate the evolution of steroid binding to nuclear receptors.
|
| Tipo: |
Manuscript
|
| Identificador: |
http://precedings.nature.com/documents/3316/version/1
oai:nature.com:10101/npre.2009.3316.1
http://hdl.handle.net/10101/npre.2009.3316.1
|
| Fonte: |
Nature Precedings
|
| Direitos: |
Creative Commons Attribution 3.0 License
|
